Detection of human papillomavirus genotypes BY pyrosequencing method

Human papillomavirus (HPV) is the major cause of cervical cancer. The aim of this study was to detect HPV genotypes by pyrosequencing method among women attending the Department of Gynecology, Faculty of Medicine, Çukurova University. Cervical cytology samples were collected from 1145 women aged 20-65 years old. HPV Sign PQ genotyping test (Diatech Pharmacogenetics, Italy) based on pyrosequencing method was used for HPV genotyping. Pyrosequencing was performed on PyroMark Q24 instrument (Qiagen). The prevalence of HPV infection was 20% (229) in total 1145 women. Single HPV infection and multiple HPV infection was found in 11.8% (136) and 8.2% (93) of the study population, respectively. HPV 16 was the most prevalent HPV genotype (9.4%), followed by HPV 18 (4.8%), HPV 56 (4%), HPV 51 (2.5%), HPV 33 (2.2%), HPV 39 (1.9%), HPV 31 (1.7%), HPV 35 (1.7%), HPV 58 (1.1%), HPV 59 (0.9%), HPV 68 (0.5%), HPV 45 (0.4%), HPV 66 (0.4%) and HPV 52 (0.2%). HPV 16 and HPV 18 found as the most common HPV types in this study are targeted by prophylactic HPV vaccines. The diagnosis of HPV genotypes by pyrosequencing test which is a new generation sequencing method would contribute to identify women at risk for cervical cancer.