The effects of hypericum perforatum on fractured bonehealing in rats (An experimental study)

Aim: Nowadays, rat standard fracture models are widely used in the investigation of fracture healing and the effectiveness of different surgical methods and medical treatments. In the literature, although there are many studies in which a standard fracture model was created and healing was examined, the number of experimental studies investigating the effects of Hypericum perforatum (HP) on fracture healing is quite insufficient, and this issue has not yet been evaluated in all aspects. Therefore, in this experimental study, it is aimed to investigate the effect of Hypericum perforatum on fracture healing radiologically and histopathologically. Materials and methods: In Aydın Adnan Menderes University and Research Hospital Animal Experiment Laboratory and Orthopedics and Traumatology Clinic, 25 Sprague Dowley male rats weighing 500-600 g were divided into three groups as Control and Experiment (Hypericum perforatum low and high dose). Standard open fractures were created in the right femurs of all rats under general anesthesia. Starting from the same day after the fracture, until the animals were sacrificed, St. John's Wort Extract-St. St. John's Wort Capsules (Balen, Turkey) was applied (1). They were acrificed 28 days after fracture formation, and the union tissues were examined radiologically and histopathologically. In the statistics performed in our study, the evaluation results here were scored in the AP, lateral AP + lateral graph scoring by the 1st orthopedist and the 2nd orthopedist. The average of low and high dose drug group scoring was found to be higher than the average of the control group, but it was not statistically significant (p> 0.05). After the histological evaluation, the mean score was higher in the control group. But statistically, no significant difference was found as a result of histopathological examination (p> 0.05). Findings: In our study, a statistical study of the scoring of AP, lateral, AP + lateral radiographs evaluated by the 1st orthopedist and the 2nd orthopedist was performed. The average of low and high dose drug group scoring was found to be higher than the mean of the control group, but it was not statistically significant (p> 0.05). After the histological evaluation, the mean score was higher in the control group. Statistically, no significant difference was found as a result of histopathological examination (p> 0.05). Conclusion: As a result of our study, according to the data obtained from radiological and histopathological tests, a statistically positive or negative effect of Hypericum perforatum on fracture healing could not be demonstrated. In this study, it was observed that Lane and Sandhu fracture union radiological scoring system may not be in parallel with histological data.