Identification of on intestinal microbiota associated with irritable bowel syndrome

The balance of microbial communities in the human gut is critical to maintaining good health. Irritable bowel syndrome (IBS) is a common digestive disorder characterized by chronic and recurrent abdominal pain and altered bowel habits. Genetic, epigenetic, and gender-related factors are known to influence the function of the higher nervous and immune-endocrine systems, as well as the regulation of brain-gut physiology, bile acid production, and absorption. The objective of this study is to investigate the diversity of gut microbiota in patients with IBS. The study aims to determine the impact of changes in microbial diversity (low or high) on IBS symptoms, as well as the effects of diseases that patients have on microbiota and IBS. A gastroenterologist at Balcalı Hospital collected samples by colonoscopy biopsy, obtaining a total of 10 gut samples from IBS patients. Bacterial biodiversity was analyzed using Illumina-based next-generation sequencing methods. Samples from 10 patients with IBS symptoms were compared, and bacterial communities were analyzed to interpret the relationships between symptoms.