Examination of sperm dna integrity and oxidative damage in infertile patients with idiopathic and non-idiopathic asthenozoospermia

It was aimed to examine the integrity of sperm DNA and oxidative damage in idiopathic and non-idiopathic asthenozoospermic infertile patients. Spermiogram evaluations and viability tests were performed on 20 normozoospermic fertile, 20 idiopathic asthenozoospermic and 20 non-idiopathic asthenozoospermic infertile patients. DNA fragmentation was assessed by the TUNEL method. For DCFH-stained sperm, flow cytometric analysis was performed for the examination. Although there was no significant difference in sperm concentration, totalmotility, progressive motility, viability and normal morphology evaluations between idiopathic and non-idiopathic asthenozoospermic infertile groups, there was a statistically significant difference between these groups and normozoospermic fertile group (p≤ 0,05). The highest statistical significance was found in the idiopathic asthenozoospermic infertile group and the lowest in the normozoospermic fertile group for abnormal chromatin condensation, DNA fragmentation and intracellular high ROS values, and there was a significant difference between all groups (p≤0,05). As a result; According to the results of this study; the sperm DNA integrity and ROS of the group of idiopathic asthenozoospermic infertile patients in male infertility were found to be more affected by low sperm motility. It is thought that the results obtained may be a useful diagnosis for the evaluation and treatment of idiopathic astenoozoospermic male infertility with the help of analysis of sperm DNA integrity and oxidative damage. Keywords; Idiopathic asthenozoospermic (IDA) , ROS, sperm DNA integrity, flow cytometry, TUNEL.