Investigation of the effectiveness of alpha-terpineol in the in vitro lipopolysaccaride stimulated liver inflammation model

Due to the antioxidant properties of terpineols, biological effects such as anticancer, anticonvulsant and antiulcer have been revealed in many studies. The aim of the research was to investigate the biofunctions of α-terpineol such as anti-inflammatory and antiapoptotic in the inflammatory model created by applying LPS isolated from Ecoli to liver cells at an effective concentration. For this purpose, HepG2 cells of human origin from continuous cell lines were selected as the test material. LPS and α-terpineol, selected as the active ingredient, were commercially available and applied to the cells at different concentrations for 24 hours. Effective concentrations of both active ingredients were carried out by cell viability tests. TNF-α, IL-1β and IL-10, caspase 3, Bax and Bcl-2 gene expression levels were investigated by real-time quantitative polymerase chain reaction (qRT-PCR). According to the results obtained in the study, different concentrations of LPS were applied to the cells and 50 ng / ml concentration that caused 11.5 % cell loss compared to the control group was selected for modeling. However, when used together with LPS, αterpineol was found to prevent cell loss at a concentration of 10 μM at a rate of 2.12%. When gene analysis was evaluated in the study, it was found that LPS increased gene expressions of TNFα and IL-1β, and α-terpineol application reversed this situation. The other IL-10 gene expression was also suppressed by LPS and it was significantly stimulated by α-terpineol. Besides, in the study, it was found that LPS increased caspase 3 and Bax gene expressions, while α-terpineol significantly suppressed this stimulation. In addition,Bcl-2 gene expressions were found to be suppressed by LPS and α-terpineol application stimulated this gene expression. With the data obtained from the study, it was seen that the short-term and low proliferative concentration of monoterpene α-terpineol may be an alternative treatment agent especially in pathogen-induced liver disorders. However, it was concluded that in vivo and more advanced studies are needed to fully illuminate the subject. Keywords: Lipopolysaccharide, inflammation, α-terpineol, apoptosis. Investıgatıon of the Effectıveness of Alpha-terpineol in the In Vitro lipopolysaccaride Stimulated Liver Inflammation Model Due to the antioxidant properties of terpineols, biological effects such as anticancer, anticonvulsant and antiulcer have been revealed in many studies. The aim of the research was to investigate the biofunctions of α-terpineol such as anti-inflammatory and antiapoptotic in the inflammatory model created by applying LPS isolated from Ecoli to liver cells at an effective concentration. For this purpose, HepG2 cells of human origin from continuous cell lines were selected as the test material. LPS and α-terpineol, selected as the active ingredient, were commercially available and applied to the cells at different concentrations for 24 hours. Effective concentrations of both active ingredients were carried out by cell viability tests. TNF-α, IL-1β and IL-10, caspase 3, Bax and Bcl-2 gene expression levels were investigated by real-time quantitative polymerase chain reaction (qRT-PCR). According to the results obtained in the study, different concentrations of LPS were applied to the cells and 50 ng / ml concentration that caused 11.5 % cell loss compared to the control group was selected for modeling. However, when used together with LPS, α-terpineol was found to prevent cell loss at a concentration of 10 μM at a rate of 2.12%. When gene analysis was evaluated in the study, it was found that LPS increased gene expressions of TNFα and IL-1β, and α-terpineol application reversed this situation. The other IL-10 gene expression was also suppressed by LPS and it was significantly stimulated by α-terpineol. Besides, in the study, it was found that LPS increased caspase 3 and Bax gene expressions, while α-terpineol significantly suppressed this stimulation. In addition,Bcl-2 gene expressions were found to be suppressed by LPS and α-terpineol application stimulated this gene expression. With the data obtained from the study, it was seen that the short-term and low proliferative concentration of monoterpene α-terpineol may be an alternative treatment agent especially in pathogen-induced liver disorders. However, it was concluded that in vivo and more advanced studies are needed to fully illuminate the subject. Keywords: Lipopolysaccharide, inflammation, α-terpineol, apoptosis.