Clonal propagation of plum (Prunus domestica L.) rootstock by in vitro shoot tip method.

Clonal Propagation of Plum (Prunus domestica L.) Rootstock by İn vitro Shoot Tip Method. It is known that the cultural history of plum goes back to 2000 years before and it was learned that there were many beautiful and various cultural varieties in those times. Eight different homeland regions (gene centers) were determined for Plum in the world. Anatolia, one of these homeland regions, is shown as the gene center of the plum.The plum is typically vegetatively propagated by seed and is done by grafting.Although cutting replication is preferred, season-dependent occurrence is troublesome and challenges arise with aspects for require large areas to propagation. Shoot tip explants were subjected to surface sterilization in the first stage; commercial NaOCl (containing 5% NaOCl-ACE) was kept at different concentrations and different durations. As plant growth regulator for shoot formation (BBD); 0.5 and 1 mg / L Benzylaminopurine (BAP) together with 0.25, 0.5, 1 mg / L of 3 different concentrations of 5 types of auxin were used. MS (Murashige Skoog) was used as the medium, 30 g / L sucrose, 8 g / L agar was added and the pH was buffered to 5.7-5.8 and autoclaved at 121◦C for 15 minutes. Sterile explants were sown in the medium and taken to the culture room at 22 ± 1 ◦C for 16 h light and 8 h dark. Observed shoot shoot at certain intervals, the number of leaves and length were recorded. The data were subjected to ANOVA. Sterilization was best obtained in the study in which 30 sn of 70 % EtOH and 10 min 5% Commercial NaOCl (containing 5% NaOCl-ACE) + 2-3 drops Tween 80 were treated. The ability to generate shoots was most observed at concentrations of BAP NAA of 0.5 mg / l and with concentrations of BAP + IBA of 1 mg/l. When the number of leaves of the shoots were examined, the maximum number of leaves was observed in the medium containing 0.5 mg / L BAP + 0.25 mg / L IBA.Rooting stage; ½ MS + full sucrose with two different protocols and 0.2, 0.3, 0.5, 1, 2 mg / L IBA and ½ MS + ½ S and 0.2, 0.3, 0.5, 1, 2 mg / L ' IBA concentrations were used. The maximum number of roots was recorded in 1 mg / L IBA medium containing 1/2 MS + ½ S. In the last stage, the rooted plants, which have completed their development, were taken into acclimatization environments and their development was observed. Key Words: Prunus domestica L., micropropagation, , plum, in vitro