İnsan kaynaklı Lactobacillus reuteri'nin moleküler ve biyokimyasal karakterizasyonu

According to clinical research intestinal probiotics are live microorganisms that provide health benefits when ingested in sufficient quantities and their main functions were related to modulating and maintaining the health of the intestinal tract. Probiotics have several novel applications because of their special ability to produce bioactive molecules including vitamins, bacteriocins, 3-indoleacrylic acid, Indole-3-aldehyde, short-chain fatty acids, exopolysaccharides, peptides, and proteins. These substances are essential in giving the host a variety of health advantages. Although Lactobacillus (L.) species are commonly used as probiotics, only limited data are available for the probiotic properties of human originated Lactobacillus reuteri. This study aims to characterize human originated L. reuteri for its probiotic potential according to the international guidelines for the evaluation of probiotics. In the present study, the probiotic potential of L. reuteri isolated from healthy humans was evaluated. The probiotic properties of L. reuteri were tested for aspects of antimicrobial activity, antibiotic susceptibility, adhesion and aggregation ability, bile salt tolerance low pH resistance and antimicrobial peptide production capacity. While clear zones of inhibition test results indicated that the cell free supernatant and cell lysate of L. reuteri had antagonistic activity against three pathogenic bacteria, Staphylococcus aureus, Yersinia enterocolitica, Salmonella typhimurium, the antibiotic test results showed that L. reuteri was sensitive for Erythromycin, but resistance for Neomycin and Kanamycin. On the other hand, L. reuteri showed a strong ability to adhere to pathogenic bacteria as well as self-binding because it has a high sedimentation rate. Furthermore, L. reuteri showed a high survival rate under acidic and bile salt containing environment that means gastrointestinal tract conditions. While in-silico analysis for BSH screening results revealed the existence of a protein acquired using specifically designed primers, further research is needed to identify BSH in this strain. Obtained results show that such properties of the human originated L. reuteri may be used as probiotics in the healthcare sector and various food products.