The Effects Of Hypericum Heterophyllum Vent. On Micronucleus, Mitotic Index And Replication Index In Perıpheral Blood Lymphocytes

Hypericum species have been used in traditional medicine in Turkey for thousands of years. In this study, in order to determine the genotoxic effect of Hypericum heterophyllum, human lymphocytes was incubated with the aqueous extracts. The increasing extract concentrations of Hypericum heterophyllum induced micronucleus rate. The rise of micronucleus shows that Hypericum heterophyllum at high concentrations may become carcinogenic and genotoxic. Mitotic index frequencies and replication index values increased with increasing extract concentrations of Hypericum heterophyllum too. The results indicate the cytotoxic effects as well as proliferative effects and suggest that the extracts of the compounds exhibit cytotoxic properties as well as mitotic and proliferative properties. The positive correlation was observed between micronucleus and age. Also, the positive correlation was observed between micronucleus rates of female and male. The negative correlations were observed between mitotic index and age, replication index and age. Further studies will be needed to determine the effects of the main bioactive components isolated from this species on micronucleus, mitotic index and replication index.