Gene expression profile in the inverted papillomas and SCC cases

This is an experimental study aiming to determine the genes whose expression changes at the ribonucleic acid (RNA) level, in the sinonasal squamous cell carcinoma (SCC) transformation of sinonasal inverted papilloma (IP). This study was carried out with Başkent University Medical Biology, Medical Genetics, Otorhinolaryngology, Pathology Departments and Dr. Abdurrahman Yurtaslan Ankara Oncology Training and Research Hospital Pathology and Otorhinolaryngology Departments contribution. RNA was isolated wtih commercially available kit from the paraffin embedded tissues (FFPEs) of 16 patients, over 18 years, diagnosed as IP, sinonasal SCC, and concha hypertrophy (6 IP, 5 sinonasal SCC, 5 concha hypertrophy) in the past five years. Using appropriate and sufficient amounts of RNA, a microarray method was used to obtain information of the gene expression profiles of all three tissues's entire genome. This information was compared using analysis programs among the three tissues. When comparing IP and sinonasal SCC tissues, the following genes with significant expression changes were identified: CYP3A7, KRT4, FA2H, BNIP3, MMP11, COL1A1, CCND2, IGF2BP3, SH3BGR, SERPINB3. As a result, both gene and protein expression related to the altered expression of CYP3A7, KRT4, FA2H, BNIP3, MMP11, COL1A1, CCND2, IGF2BP3, SH3BGR, SERPINB3 and THBS2 can be studied together to confirm the results of future studies on IP etiology and carcinogenesis.