Investigation of effects of extracts from digitalis lamarckii ianina (foxglove) on xanthine oxidase enzyme activity

This study focuses on xanthine oxidase (XO), a key enzyme generating reactive oxygen species (ROS) in biological systems. Under specific stress conditions, XO transforms into an oxidant enzyme, potentially causing health issues such as kidney stones and gout when uric acid levels are high. The primary objective of this research is to use plant extracts to block the xanthine oxidase enzyme. Hypoxanthine is converted to xanthine by the xanthine oxidase enzyme, and xanthine is then converted to uric acid, which is excreted by the kidneys. The (aim?) of this research is to investigate the ability of the plant extract Lamarck's Ianina in inhibiting the xanthine oxidase enzyme by taking boiled extracts obtained from the root, stem, leaves and flowers of the plant Digitalis Lamarckii and dissolving them in methanol, hexane, chloroform and acetone. The inhibitory effects of Digitalis lamarcke on xanthine oxidase enzyme have been demonstrated. The results showed its effectiveness in 19 out of 20 samples due to its ability to inhibit xanthine oxidase. This means that the plant Digitalis lamarcke ianina has a very great ability to influence the enzyme xanthine oxidase. A research was conducted on the chemical components found in the Lamarckii Ianina plant and the xanthine oxidase enzyme. A chemical interaction took place between them, suggesting the potential development of new medications for treating conditions linked to elevated xanthine oxidase enzyme activity, such as gout and related infections, by inhibiting the enzyme's activity.