A new approach to the evaluation of hemostasis in dogs with heart failure: platelet proteomic profile

Heart failure can cause haemostatic complications due to platelet over- activation. There is not enough information on the molecular relationship between hemostasis and cardiovascular diseases in veterinary medicine. Thus, in this study, it was aimed to elaborate hemostasis (coagulation) changes with the platelet proteomic profile in dogs with naturally occurred heart failure. The material of this study consisted of totally 20 dogs with different breed, age, and sexes. Two different group were designed; control (n=10) and test groups (n=10). Based on the physical, laboratory and cardiologic examination results, dogs were included into control (healthy) group, or dogs with heart failure were enrolled into test group. Platelet isolations were performed from each dog, and platelet pellets were stored -80 C until analysed. Platelet proteomes were identified by use of UPLC- ESI/QTOF/MS method. Platelet proteomes were matched with the previously described proteins for Canis lupus familialis, and compared to the control, at least P <0.05 level and 1.2 fold change of proteomes was considered statistically significant. Compared to control group, guanine nucleotide-binding protein subunit alpha-11, apolipoprotein C-III, apolipoprotein A-II and clusterin levels increased whereas CXC-motif chemokine-10, cytochrome-C-oxidase subunit-2, cathepsin-D, serine/threonine- protein phosphatase PP1-gamma catalytic subunit, creatine kinase B-type and myotrophin levels decreased in test group. As a result, each platelet protein identified in the present study could be a potential biomarker in the process of heart failure and therefore these proteins need validation with field studies. Key Words: Dogs, heart failure, hemostasis, platelet proteomic