Investigation of radiopharmaceutic potential of a new radiolabeled graft polymer for using in the therapy and in the molecular imaging on albino wistar rats

With current parallel breakthroughs in molecular understanding of various diseases and controlled manipulation of materials at the nanometric scale, nanotechnology offers marvelous promise in therapy, prevention and diagnosis of various complex diseases including cancer. Thus, a budding interest in nanotechnology has been generated remarkable number of advancements in recent years with a main focus on current cancer therapy. Grafted polymers in the biomedical field due to its unique physicochemical advantages and increasing demands are today very widely for used. In designing grafted and crosslinked polymers for biomedical and pharmaceutical applications, it is essential to design these polymers with desired tunable physicochemical and biomimicking properties. For example, for drug delivery application, the important design criterion is to achieve maximum therapeutic effi cacy of encapsulated drugs with minimum toxicity. Uptake of radiopharmaceutical in the cell for diagnosis and treatment of tumours is highly important. In order to do it, an appropriate radioactive tracer is required to enter the cell. In the case of radioiodination of poly(HEMA) grafted with L-Lysine, it can be possible for radioiodine to deliver the cell in this condition. Thus, depends on radioactive iodine which is used, it's going to be possible for poly(HEMA) grafted with L-Lysine which is radiolabeled with radioactive iodine to be used in imaging and therapeutics in Nuclear Medicine as a novel radiopharmaceutical. In order to increase the efficiency and selectivitiy of this nanopolymer in medical applications, it will be given the magnetic property to nanopolymer. In this study, firstly, 2-hydroxyethyl methacrylate (HEMA) will be polymerized in the presence of Fe3O4 particles and then new generation nanoparticles are going to be obtained. After that, L-Lysine aminoacid will be grafted to polymer. In characterization studies of new generation magnetic m-graft-Lys-poli(HEMA) nanoparticles which is synthesized, FTIR, Zeta dimension, Zeta potential and SEM methods are planned to be used. Optimization of radiolabeling with radioiodine of new generation magnetic grafted polymer is going to be carried out by TLRC and HPLC methods. At the last stage of the project which is presented, as application platform, it's planned to investigate the in vivo efficacy of new generation nanoparticles by using experiment animals. In in-vivo stage of the project, scintigraphy and biodistribution studies are going to be carried out on Albino Wistar rats. It's going to be determined the organs in which m-graft-Lys-poli(HEMA) is specific with scintigraphy and biodistribution studies. Thus, it's going to be determined the organs which are retained by radiolabeled nanoparticles and the retention time of radiolabeled nanoparticles in these organs, and as a result of this it's going to be obtained important results for cell studies which is planned to be carried out in the future. Keywords: HEMA, L-Lysine, Grafted Polymer, Nanoparticle, Cancer, Radioiodne, Tc-99m