investigation of oxidan/antioxidant status treated with (CCl4) in rat heart tissue

CCI4 is a typical toxic agent, and causes production of free-radical.In this study, the heart tissues of rats given CCl4 in the metabolism of free radical antioxidant enzymes superoxide dismutase (SOD), glutathione peroxidase (GPx), glutathione-S-transferase (GST) and catalase (CAT) enzyme activities, heart tissue glutathione (GSH) with levels of lipid peroxidation product, malondialdehyde levels were investigated.Twenty male Wistar-Albino rats weighing approximately 250-300 grams were used in the study. Group?I Control, group-II Carbon tetrachloride.Group I (Control group) for 8 weeks, rats were given standard rat chow. Group II (CCl4 group) rats were given intraperitoneally in increasing doses 0.3ml/kg CCl4 for 8 weeks (1st week), 0.7ml/kg (2nd weeks) and 1 ml / kg for 6 weeks.After eight weeks, the rats were sacrificed under anesthesia, heart tissues were removed. We investigeted probably oxidant effects of CCl4 on heart tissue and antioxidant defense system of cardiac tissue, and conducted enzymatic and histological examination.In our study, GPx and SOD enzyme activities in CCl4 group were significantly increased compared with control group (p <0.05 and p <0.05). GST and CAT enzyme activity in CCl4 group decreased compared with control group. However, this reduction was not statistically significant (p> 0.05 and p> 0.05) . GSH and MDA levels of heart tissue in CCI4 group, a statistically significant increase was observed (p <0.05 and p <0.05). In parallel to these data, CCl4 group according to the control group, some histopathological changes were observed.According to the results of our experiment of tissue MDA levels increased in CCI4 group compared with control group, just as GPx and SOD activity increased. According to the results of spearmann correlation analyze strong positive correlation was found among the three of them. GSH levels of heart tissue with a strong positive correlation was found between SOD activity. GSH activity in the heart tissue with a strong positive correlation was found between CAT activity. SOD activity of CCI4 group increased, while GSH levels increased and a strong positive correlation was found between them. GPx activity in the heart and a weak negative correlation was found between CAT activity in the heart. Tissue GPx activity increased, while CAT activity decrease. GPx activity in the heart tissue with a strong positive correlation was found between SOD activity. SOD activity of CCI4 group increased, while GPx levels increased and a strong positive correlation was found between them. GST levels of heart tissue with a strong positive correlation was found between CAT activity. Tissue GST activity decrease, while CAT activity decreased.Oxidative stress may be a major mechanism for the toxicity of CCl4. In CCI4 group MDA level is higher than the control group supports this opinion. The low level of CAT activity in heart tissue, heart tissue does not show an increase of hydrogen peroxide. Because our study found high levels of GSH and GPx levels. GSH-Px enzyme need to GSH as hydrogen donor to clean peroxides . We think increased of glutathione synthetase enzyme that involved in the synthesis of GSH and (-) feedback, which controls the synthesis of GSH therfore Both GSH and GSH-Px increase in the CCI4 group. İncreased antioxidant enzyme activities, only moderate damage apparents in the histopathologic examination against oxidative stress caused by CCI4 in heart tissue, suppose that there is a strong and effective antioxidant system.