The consideration of the microorganisms isolated from respiratory tract samples of the patients with cystic fibrosis

ABSTRACT The Consideration Of The Microorganisms İsolated From Respiratory Tract Samples Of The Patients With Cystic Fibrosis Müzeyyen Selin BÜDEYRİ SAYIN Master Thesis, University of Gaziantep, Institute of Medical Sciences Department of Medical Microbiology Thesis Supervisor: Prof. Dr. Yasemin ZER 84 pages, July 2018 Cystic fibrosis (CF) is the most common genetic disorder of autosomal recessive transmission in the white race, with a frequency of 2,500 live births. In the few incidence studies conducted in Turkey are expressed as 1/3000 and, if this ratio isconsidered to be favorites of consanguinity is estimated to be higher. At the beginning of the last century, it was identified as "a childhood illness in the first year",and with the increase in knowledge about the disease, the life of the patients has been prolonged and the disease has become a disease of concern to adults. The main cause of mortality in patients with cystic fibrosis is recurrent pulmonary infections. Mucosal genetic defect causes colonization of various microorganisms first in the upper respiratory tract, then in the lower respiratory tract. This study was carried out with the aim of identifying respiratory tract pathogens detected in patients with cystic fibrosis and examining the phenotypic changes observed in these bacteria. Gaziantep University Sahinbey Research and Practice Hospital Pediatric Immunology Polyclinic was evaluated between the period of May 2016 and May 2017, and the respiratory tract samples routinely taken from cystic fibrosis patients who were under follow-up and started to follow up with new diagnosis were evaluated. In the study, 500 respiratory tract samples (throat or sputum) from 123 patients were taken. In 282 (56.4 %) of the samples, normal throat flora was detected. At least one pathogenic microorganism (232 different bacteria) was isolated at 218 (43.6 %).The most frequently isolated microorganisms are; S. aureus (96 isolates, % 18.3) and P.aeruginosa (62 isolates, % 11.8). Phenotypic differences were observed in 16 S.aureus and 24 P. aeruginosa isolated bacteria (small colony variant in Staphylococcus, mucoid colony in Pseudomonas). All isolates with phenotypic differences were found to be diagnosed at least 2 years ago. In cystic fibrosis patients, phenotypic changes occur in bacteria that colonize respiration when the disease is prolonged, which allows the immunological system to falsely escape from the immune system. Identification of these changes is important establishing protective medical approaches from the colonization. Key words: Cystic fibrosis, respiratory tract infection, P. aeruginosa, S. aureus