The comparison of resistance profiles and characterization of resistancemutations by molecular methods of resistance mutations aganist first-line andsecond-line antituberculosis drugs of multiple drug resistant mycobacteriumtuberculosis strains isolated from clinical specimens

The Comparison of Resistance Profiles and Characterization of Resistance Mutations by Molecular Methods of Resistance Mutations Aganist First-Line and Second-Line Antituberculosis Drugs Of Multiple Drug Resistant Mycobacterium Tuberculosis Strains Isolated From Clinical Specimens Aim: Multi-drug resistant tuberculosis (MDR-TB) is still an important public problem. Rapid diagnosis of the agent and determination of resistance status are critical in establishing the correct treatment protocol. This study was conducted with the aim of determining resistance mutations against first and second generation drugs in strains isolated from respiratory tract specimens and detected by conventional methods. Material and method: Adıyaman University Medical Faculty Training and Research Hospital and obtained from Dr. İsmail Ceyhan from Atatürk Chest Diseases and Thoracic Surgery Training and Research Hospital on a voluntary basis 33 MDR-MTB isolates were included in this study. After the subculture of these strains in Löwenstein - Jensen medium, DNA isolation was performed according to the boiling method. DNA isolates were kept at -40 0C until the working day. Primer sequences specific to rpoB, InhA, katG, gyrA, eis and rrs regions were used to determine isoniazid, rifampicin, quinolone and aminoglycoside resistance. Results: The positivity rate of rpoB, InhA, katG, gyrA, eis and rrs in 33 MDR-TB isolates by in-house PCR method was 27 (81.8 %), 31 (93.9 %), 25 (75.7 %), 25 (75.7 %), 20 (60.6 %) and 14 (42.4 %). Resistance mutations were not detected in susceptible isolates. Conclusion: According to the data obtained from the study, it was found that fluoroquinolone resistance mutations were higher in isolates defined as MDR-TB by conventional and molecular methods, and in-house PCR method was a useful method for rapid resistance detection. Key words: MDR-TB, Mutation, Mycobacterium tuberculosis, drug resistance, PCR