The evaluation of root canal irrigation solutions penetration to the dentine tubules

The purpose of this in vitro study was to determine the effects of 5.25% Sodium hypochloride, 0.1% Octenidine Dihydrochloride and 2% Chlorhexidine gluconate that penetrated to dentinal tubules of primary and permanent teeth on E.faecalis and C.albicans in 30 seconds, 1 minute and 5 minutes. The tooth sections were sterilized and contaminated with a mixture of E.faecalis and C.albicans strains and this procedure was carried out for 3 weeks. Contaminated dentinal surfaces were examined with SEM. After aplication of irrigating solutions to the contaminated tooth sections, neutralizers were applied for inactivation of the solutions. TSB was added to the dentine powders that were removed by round burs from specimens and they were placed into BHIB and incubated for 24h at 37°C. The colonies were counted macroscopically. There was no statistically significant difference between primary and permanent teeth with respect to the antimicrobial activity of tested solutions (p>0,05). Among the solutions that were tested against E.faecalis on primary and permanent teeth, the most effective one was found as 0.1% Octenidine Dihydrochloride of 5 minutes application (p<0,05). The effects of tested solutions on the same time durations against C.albicans showed no significant difference (p>0,05). Antibacterial activity of all tested groups and control group with saline was compared and a statistically significant difference was found (p<0,05). Key Words: rrigation solutions, dentine tubule disinfection, Octenidine Dihydrochloride. Sodium Hypocholorite, Chlorhexidine