Kolorektal kanserli hastalarda 21q11-22 bölgesi LOH analizi

Colorectal cancer (CRC) is the most common cancers in western populations. In Iraq, a developing Asian country in the Eastern Mediterranean region, these rates reach four times less than in developed countries. The pathogenesis of CRC is very complex and diverse and it's also influenced by multiple processes. The detection of loss of heterozygosity (LOH) of tumor suppressor genes and deletion chromosome associated with colorectal cancer, so the microsatellite DNA loci are useful markers for which aims in our study. The present study examined 5 polymorphic microsatellite markers in along chromosome 21q11-22 region matched in 42 patients of CRC (tumor and normal) tissues identified frequency loss of heterozygosity of each loci of markers, Comparisons between LOH frequency and some of pathological etiology of the sampled tissues were performed by fisher's exact test, P<0.05 was considered as statistically significant. We get 29 (%57, 1) patients were detected LOH from sum of 42 cases at least in one locus. The highest frequency of LOH was found on D21S1839 (%19) loci. The lowest frequency of LOH was on D21S270 (%7, 1) loci. The occurrence of LOH in region 21q11-22 was extremely significant with gender (P=0.0001). It is concluded that LOH in region 21q11-22 is found to be associated with CRC in females. Keywords: Colorectal cancer, loss of heterozygosity, chromosome 21