Investıgatıon of miR-485-3p and miR-4728-5p expressıon levels ın tumor and healthy tıssues of patıents wıth colorectal cancer

Colorectal cancer (CRC) is one of the most common types of cancer that develops due to genetic factors, environmental factors, and digestive system disorders. In particular, with recent studies aimed to understand the mechanisms of colorectal cancer and determine early diagnosis for colorectal cancer. Previous studies have reported that micro RNAs (miRNAs) regulate the expression of protein-coding genes in CRC. In this thesis, it was aimed to reveal the differences in miR-485-3p and miR-4728-5p expression levels in the tissues of CRC patients. This study has received approval by local ethical committee of Gaziantep University, and all participants signed the informed consent to participate in this research. Tumor and adjacent healthy tissues of a total of 59 patients diagnosed with CRC were collected from Department of General Surgery, Gaziantep University-Turkey. In the present study, RNA from both tumor and adjacent healthy tissues of colorectal cancer patients were extracted and synthesized the cDNA. Then, the expression levels of miR-485-3p and miR-4728-5p were determined by quantitative Real-Time PCR. As a result, the expression levels of miR-485-3p and miR-4728-5p were determined significantly downregulated in colorectal cancer tissues compared to adjacent healthy tissues (p˂0,05). Consequently, it has been concluded that miR-485-3p and miR-4728-5p may be important biological markers as tumor suppressors in the CRC carcinogenesis. Key Words: Colorectal cancer, expression level, miRNA 485-3p, miRNA 4728-5p, RT-PCR