Cellulase isolation and characteri̇zation from microorganisms in kombucha simbiosis

Samples were isolated from kombucha tea simbiosis and highest cellulase producers were selected and identified as Candida sp and Bacillus sp. Maximum enzyme activity measured at 9.5 and 60°C for Candida sp SNK-5, Bacillus sp SAK-5 showed maximum enzyme activity at 9.0 and 40°C. Cellulase of Candida sp. SNK-8 had 100 % residual activity at pH 9. Cellulase of Bacillus sp SAK-5 had 100 % residual activity at pH 7.0. Cellulase of Candida sp. SNK-8 was retained 100% residual activity at 30°C and it has shown 85% residual activity at 40°C for cellulase activity. Cellulase of Bacillus sp. SAK-5 has shown %100 residual activity at 40°C and stable between 30°C -50°C but activity decreased at above 50°C. CaCl2, Na2SO3 and SDS stimulated enzyme activity. This study shows that Bacillus sp. SAK-5 and Candida sp. SNK-8 are suitable for commercial cellulase preparation. If pH stability and alkaliphilic properties are considered, these cellulases have potential to be used for most industrial applications.