The investigation of the pathogenesis of kidney levels in canine visceral leishmaniosis

Canine Visceral Leishmaniozis (KVL) is a protozoal infection with zoonotic character which processes with lymphadenitis, osteomyelitis, hepatitis, dermatitis and nephritis in domestic and wild carnivores. It is very important to determine the amastigotes in infected tissues of Leishmaniozis. Immunohistochemical studies with Leishmania-specific polyclonal / monoclonal antibodies reveal the expression of amastigote antigens and thus a definite diagnosis of the disease is performed. This study aimed to illuminate the possible immunopathologic mechanisms of renal damage in KVL. In this study, the kidneys of dogs cases diagnosed as KVL histopathologically / immunohistochemically were examined at Adnan Menderes University Faculty of Veterinary Medicine Department of Pathology between 2002 and 2015. For this purpose, Leishmania amastigot antigen, IgG and Complement-3 (C3) deposits were specified immunohistochemically in the kidney tissues of infected dogs by determining histopathological lesions in kidney tissue of the cases. Immunohistochemically, amastigote positive reactions were obtained in lymphocyte and kidney interstitium in macrophage cytoplasm. In addition to this, IgG positive reactions deposited in the kidney glomerulus basal membranes revealed C3 positive reactions deposited in the kidney glomerulus basal membranes and tubulus basal membranes. As a result, this study revealed that IgG and C3 immunohistochemical expressions are intense and severe compared to control group of animals in the form of immunocomplex deposits of infected dogs in kidney tissue, suggesting that KVL is mediated by immunocomplexes of the pathogenesis of renal lesions. Key Words: Leishmaniosis, Ig G, Complement 3, histochemistry, immunohistochemistry.