Paraoxonase-1 gene ın patıents wıth chronıc obstructıve pulmonary dısease ınvestıgatıon Q192R and L55M polymorphısms

The effect of increased oxidative stress in development of chronic obstructive pulmonary disease (COPD) is well known. One of the antioxidative systems against oxidative stress in human body is paraoxonase (PON) enzyme that protects low density lipoproteins (LDL) against oxidation. In this study we aimed to search the polymorphisms on PON1, Q192R, L55M genes of patients with COPD.The DNAs extraction was obtained from blood samples of the 50 patients pastly diagnosed with COPD and 50 patients as control group who were presented to emergency clinic. Genotypes were obtained with polymerase chain reaction (PCR) and AIw I and Hsp92II restriction enzymes were used for Q192R and L55M polymorphisms, respectively. Analysis of data was done with Chi-Squire and Fisher?s exact tests.A statistically significant difference regarding Q192R polymorphism was found between COPD patients and control group (P = 0.05). There was no statistically significant difference about L55M polymorphisms in patient and control groups (P > 0.05). There was a significant correlation with Q192R polymorphism on PON1 gene and cigarette usage however other risk factors did not show significant correlation with this polymorphism. Though L55M polymorphism had significant correlation with family history and tuberculosis, no significant correlation was found with other risk factors.The importance of our study is due to it is the first study in literature searching Q192R and L55M polymorphisms on PON1 gene in COPD patients. We believe that more studies are needed in this subject.Key Words: COPD, paraoxonase, polymorphism, acute attack