Quantification, method validation and analysis of essential oil componennts of some common species of labiatae family by GC-MS

Aim: In this thesis, the essential oils of some species collected from the Eastern and Southeastern Anatolia regions of Turkey and widely used as tea and spice among the people were prepared with the Clevenger apparatus. It is aimed to develop and validate a GC-MS quantification method that includes special components are provided from the market to these species. In addition, the contents of these species in terms of these components were determined. Material and Method: A GC-MS method was developed and validated with the components which including α-Pinene, camphene, α-fellandrene, p-cymene, limonene, eucalyptol (1,8-cineol), β-ocimene, γ-terpinene, linalool, camphor, citronellal, menthone, borneol, menthol, α-terpineol, cis -geraniol, pulegon, L-carvone, linalyl acetate, bornyl acetate, thymol, carvacrol, β-caryophyllene. Essential oils of some species were obtained by hydrodistillation method using Clevenger apparatus. Then, the contents of these essential oils were determined by GC-MS. Results: It was seen that, the common main component's in all studied Thymbra species were carvacrol, in all Salvia and Mentha species were eucalyptol (1,8-cineol), in all Origanum species were p-cimen, and in all Lavandula species were linalool found.It was detected that, Thymus pubescens var. crateicola is rich in thymol, Cyclotrichium origanifolium is rich in pulegon, Achillea vermicularis is rich in eucalyptol, Teucrium polium is rich in β-caryophyllene, Zizphora clinopdioides is rich in pulegon components. Conclusion: The major components of the essential oils of some species of Thymus, Thymbra, Salvia, Satureja, Lavandula, Origanum, Mentha, Achillea, Teucrium, Ziziphora, Cyclotricium, Stachys which are grown in the Eastern and Southeastern regions of our country and are widely used among the people, were determined and analyzed in terms of their potential to be source species. It was found that especially Thymbra spp. carvacrol, Cyclotricium origanifolium spp. pulegon and Lavandula spp. can be source species for linalool components. Key Words: Labiatae, Thyme, Origanum, Essential oil, GC-MS, Thymol, Carvacrol, Pulegone, Linalool, Method validation.