In Vitro investigation of the effects of laser on platelet rich fibrin

The aim of this study was to evaluate the amount of chemical degradation and fibrin network structures of laser-treated and non-laser-treated L-PRF and T-PRF membranes by SEM and light microscopy using the biostimulative effect of laser. Our study was performed on 15 systemically healthy individuals and four L-PRF and four T-PRF membranes obtained from each individual. Two of the four membranes was treated with a diode laser device at a wavelength of 980 nm and a power of 0.5 W in continuous mode for 3 minutes at a distance of 1-2 mm. The other two membranes were not lasered. One of the laser treated L-PRF and T-PRF membranes were cut in half and stored under appropriate conditions for histological examination and SEM analysis. The other membrane was separated for degradation. The same procedures were performed for L-PRF and T-PRF membranes without laser treatment. According to the results of our study, laser treated L-PRF and T-PRF membranes showed lower degradation percentages compared to non-laser treated membranes, but this difference did not reach statistical significance (p>0.05). However, when laser treated L-PRF and T-PRF membranes were compared, the degradation percentage was significantly higher in L-PRF membrane (p<0.05). Histologic examination showed that the fibrin network structure of the laser-applied L-PRF and T-PRF membrane groups was significantly denser than the non-laser-applied groups (p<0.05). SEM analysis revealed that the fibrin network was denser, thicker and more complex in the laser-treated L-PRF and T-PRF membrane groups. In our study, the biostimulative effect of laser increased the fibrin network thickness, cross-link structure and density of L-PRF and T-PRF membranes. When the degradation percentages on the membranes were evaluated, no significant difference was observed between the groups