The investigation of the effect of NAD, H2O2 and weak magnetic field on the antibacterial mechanism of isoniazid (INH) that first line antibiotic against M. tuberculosis agent

The Investigation of the Effect of NAD, H2O2 and Weak Magnetic Field on the Antibacterial Mechanism of Isoniazid That First Line Antibiotic Against M. tuberculosis Agent Aim: Nearly one-third of the world's population is infected with Mycobacterium tuberculosis bacilli. Each year around 2 million people in the world have died because of that disease. Although isoniazid is used as the most common and effective drug in therapy, mechanism of action of isoniazid is not fully elucidated. Moreover, electromagnetic field sources have a lot of positive and negative effects on health. WHO has accepted low frequency electromagnetic fields as a "Class 2B possible carcinogenic effects". In our study, we aimed to investigate the effects of isoniazid mechanisms with added NAD and H2O2 with a weak magnetic field application. Material and Method: In this study, at varying concentrations of NAD and H2O2 were added to different groups individually and in combination, and changes made on the mechanism of drug evaluated. An experiment group were exposed to 5 mT, 50 Hz magnetic fields daily 4.5 hours (total 45 hours for 10 days). Agar proportion method was used to evaluate the results. Resistant strains was demostrated by PCR-RFLP method. Results: At the end of the experiment, magnetic field application did not cause any change on the number of colonies both sensitive and resistant strains in comparation to control group (p>0,05). Combined addition of 10 µM NAD and H2O2 to media containing isoniazid for 10 days dramatically reduced the number of colonies in the group (p<0,05) and increase the effectiveness of the drug. From this point, the most effects were measured in the group of 100 µM added by adding varying concentrations of NAD and H2O2. Conclusion: Especially with the addition of 100 µM NAD and 100 µM H2O2 together was found to increase isoniazid activity as dramatically, also it was determined that weak magnetic field application does not change the efficacy of the drug. Key Words: Drug resistance,isoniazid, magnetic field, mechanism of action, Mycobacterium tuberculosis.