The effect of carnosic acid on semen freezability in Malakli dogs

The objective of the study was to determine the effect of carnosic acid, extracted from rosemary (rosmarinus officinalis), to Malakli Shepherd dog semen motility, morphology, viability and membran integrityparameters after frozen-thawed prosess. Five male animal were used in this study and the sperm rich portion of the ejaculates were mixed and divided in to five equal groups. Each group extended with either tris as a control group and containing carnosic acid 10 µM, 50 µM, 100 µM, 200 µM. Following equilibration for one and a half hours, the straws were frozen in nitrogen vapor and then stored in liquid nitrogen. Later, the frozen straws were thawed in a water bath for motility, morphology, viability and membran integrityparameters examination. The highest motility and H-E test results were detected in 10 μM group at 65.00±2.23 % and 69.83±0.70 % respectively, the lowest abnormal sperm rate were detected in 10 μM group at 9.50±1.52 % and the differences in these groups (p<0.05) were statistically significant compared to the control group. With regard conclusion, it was determined that the doses of 10 μM of carnosic acid added to extender in freezing of Malakli dog sperm showed a protective effect compared to the control group in terms of motility, spermatozoon morphology, membrane integrity and viability.