Research of the microwave hyperthermia characterization of the magnetic nanoparticle-chitosan complex

The application of hyperthermia by induction of Magnetic Nanoparticles (MNPs) presents alternative approaches for diagnosis and treatmen in the clinic. Magnetic nanoparticle hyperthermia (MNPH) can selectively heat the targeted malignant cells while preserving healthy tissue. However, being able to detect the characterizations of MNPs is important to be able to identify the matter. The aim of this thesis the matter characterization of synthesized C-MNP and the MW hyperthermia thermal efficiency of C-MNP on healthy breast and tumor equivalent tissue were investigated. This study was carried out in two main stages. In the first stage of the study, Fe3O4 nanoparticles (C-MNP) coated with chitosan were synthesized using the Ionic Gelation Nanoprecipitation Technique and matter characterization studies were carried out with DLS, ZetaSizer, DSC, FTIR, XRD and SEM devices. In the second stage, for thermal characterization, the MW application was imaged with a thermal camera, and the temperature was recorded with the Pico TC-08 Thermocouple device. The obtained numerical data were evaluated by statistical analysis. Groups were formed as Healthy Tissue, Tumor Tissue, Tumor Tissue+MNP, Tumor Tissue+C-MNP and were exposed to MW during the same time period. According to DLS results, the sizes of Fe3O4 and C-MNP nanoparticles were found to be 571.5 and 665.9 nm, respectively and their Zeta Potentials were 30.4 and -15.6 mV, respectively. According to the XRD analysis results of C-MNPs, no sharp peaks of Fe3O4 nanoparticles were observed. However, according to the results of the FTIR analysis, it was observed that the peak observed around 500-790 cm-1 corresponds to the vibration of the Fe-O bond and this proved the presence of Fe3O4 in C-MNP. As a result of DSC analysis, an endothermic peak corresponding to the melting point of C-MNPs at 97,14 °C was observed. When the size analysis results of C-MNPs were investigated by SEM, the size of C-MNPs was found to be 24.87±11.5 nm. According to the thermal characterization results, statistical significance was found in the comparison between Healthy Tissue and Tumor Tissue and other groups in temperature measurements made with the Thermocouple device (p=0.0015; p<0.0001). In the thermal camera results, it was observed that there was statistical significance in the comparison between the groups (p<0.0001). As a result, it was predicted that the C-MNP complex was successfully formed and appropriate for clinical hyperthermia treatment with MW application. Animal studies are needed to support this prediction.