Investigation the apoptotic effects of sinarin compound on breast epithelium and cancer cells

Investigation the Apoptotic Effects of Sinarin Compound On Breast Epithelium and Cancer Cells Aim: it is suggested that sinarin, a polyphenol that is highly involved in artichoke, has a protective effect against oxidative stress. In addition, the purpose of this study is to investigate the effects of sinarin on proliferation, cell death in breast cancer cells. And in this context, to clarify the role of sinarin in the cancer death process. Material and Method: In this study, MCF10 normal breast cell and MDA-MB-231, MCF7 breast cancer cells were used. With the sinarin treatment applied, cell proliferation of this treatment in cells was tested with MTT, and colony forming abilities were tested with colony forming test, western blotting and annexin PI test were used to clarify its role in cell death. Results: It was determined that cell proliferation and colony formation ability of cells in cells treated with sinarin decreased over 70 percent at concentrations of 160 µM and 180 µM, respectively, in MCF-7 and MDA-MB 231 cell lines. Apoptosis occurred in 10% in MCF10A cell, 63% in MDA-MB-231 cell and 69% in MCF-7 cell line. And all these results were analyzed using the student's T test and found statistically significant (p <0.05). Conclusion: In this study, the effects of sinarin on breast cancer cells and breast epithelial cells were analyzed. It was determined that sinarin compound had an effect on increasing apoptosis and decreasing cell proliferation. As a result of this study in which the effects expected from the drugs used in cancer treatment appeared, it was determined that sinarin substance would be a new hope for cancer treatment. Keywords: Sinarin, Epithelium, Breast Cancer