Investigation of methylation patterns in epigenetically effected genes in breast cancers by

The purpose of this study was determining the role of GSTP1 and E-cadherin genes in breast tumorogenesis that their importance in breast cancer is established. Also it was shown that E-cadherin and GSTP1 genes are methylated then the methylation relationship with tumor?s features is evaluated. The hypothesis of the study is that GSTP1 and E-cadherin genes methylation is related to tumor?s features . 50 established breast cancer samples and 50 of their adjacent normal samples were obtained with random sampling method from pathology department archieve of 9 eylul university of Turkey . After isolation of DNA, sodium bisulfite sequencing was performed in promoter regions and the comparison between tumor and normal samples was made, the relationship with tumor?s grade, stage ,? is analyzed by x2 correlation and regression programs.In E-cadherin gene, for tumor samples of 44% ( 22 in 50) were full metyhlated, 50% partial ( 25 in 50) and 6.0% (3 in 50) were non- methylated. The methylation status shows meaningful differentiation between tumor and normal samples. Generally , 94% of tumor samples were full methylated and of normal samples 76% ( 38 in 50) were non-methylated.In GSTP1 gene, for tumor samples of 28.8% (13 in 50) were full methylated, 13% (6 in 46) partial and 58.7% ( 27 in 50 ) were non-methylated. In general, from normal samples there were 87.2% non-methylated and partial methylation has seen in 12.8% of samples. There is meaningful difference in methylation status between tumor and normal samples (p=0.000).