The effect of resveratrol in myocardial ischemia-reperfusion injury; Role of bkca

The aim of the present study was to investigate the role of BKCa channel at the protective effect of resveratrol in the myocardial ischemia-reperfusion injury. Wistar rats were anesthesia with ketamine+ksilazin and hearts were put into solution after thorax was immediately opened. Finally, the hearts were mounted on the Langendorff system quickly. Left ventricular pressure was measured with a water-filled balloon. In all experiments, the infarct size and hemodynamic parameters (LVDP, LVEDP, ±dP/dt) were measured and evaluated by MP35 recording system. In the first group (control) after isolation of the hearts 10 min stabilization period followed by 20 min normal perfusion was applied. In the paxillin (3, 1, 0.3, 0.1, 0.03 µM) and resveratrol (10 µM) group, after 10 min stabilization period, heart was perfused paxillin and resveratrol separately 10 min. In the resveratrol group or paxillin group, 10 min stabilization, 10 min perfused and 10 min resveratrol or paxilllin perfused. All hearts were subjected to 30 min global ischemia followed by 120 min of reperfusion. The hearts were dyed with TTC for infarct size measurement When resveratrol was applied infarct size significantly decreased against control and paxillin. Paxillin was significantly decreased this protective effect at 3 and 1 µM concentrations. In resveratrol group LVSP, LVEDP and ±dP/dt value at 30, 60, 120 . min were not change when compared before ischemia values. Paxillin (3, 1, and 0.3 µM) markedly reduced LVSP, LVEDP and ±dP/dt value at 30, 60, 120. min when compared resveratrol. These results show that BKCa channel has potential role in resveratrol protective effect.Keyword: Resveratrol, BKCa channel, preconditioning, ischemia-reperfusion