The effect of black cumi̇n (ni̇gella sati̇va) extracts and chroni̇c exerci̇es on peni̇ci̇lli̇n i̇nduced experi̇mental epi̇lepsy i̇n Mongoli̇an gerbi̇ls

Epilepsy is a most common neurological disease which characterized repeated seizures and has adverse mental and physical effect on human. It is characterized synchronize discharge which occurs when the balance between excitatorand inhibitor mechanisms are breakdown. The aim of this studyis to examine the effects on oxidative stress and electrophysiological investigation of Nigella sativaextract and chronic exercise in gerbils which penicillin induced experimental epilepsy. In this study 40 adult male Mongolian gerbils were used. Gerbilswere divided 5groups as sham, control (saline), control (penicillin), exercise, Nigella sativa and Nigella sativa +exercise groups. Animals in exercise groups were performed treadmill exercise for 30 minutes daily and five times in a week for 2 months.Animals in Nigella sativagroups were applied with 50 mg/kg dose of 0,2 ml of Nigella sativaextract via intra gastric, five times a week for 2 months. After of exercise stage, epileptiform activity was induced by injecting 500 IU/ 2,5 μl penicillin intracortically and 120 minutes of ECoG recordings was taken via power lab system.There was no any epileptic activity in control (saline) group. Separately application of Nigella sativaand exercise showed that onset of the epileptiform activity was delayed although it was seen effect of Exercise-Nigella sativa combination is better than each individual. Application of Exercise-Nigella sativa postponed seizure and reduced spike XXIIIwave frequencies in Mongolian Gerbils. Difference between control group and others were statistically significant except some periods according to epileptiform activity amplitudes.Consequently, it was shown that application of Exercise-Nigella sativa combination postpone beginning of the epileptiform activity and decrease spike wave frequencies and epileptiform activity amplitudes. It is considered to be healthy for epilepsy patients using Nigella sativa with exercise.Key words:Epilepsy, Mongolian gerbils, Black cumin (Nigella sativa), Epileptiform activity, Electrocorticography, Oxidative Stress