Morexella catarrhalis'te bulunan muhtemel bir ABC taşıyıcısının ATPase komponenti'nin moleküler karakterizasyonu

AbstractMolecular Characterization of the ATPase component of a putative ABCtype transporter in Moraxella catarrhalisMoraxella catarrhalis is one of the major causes of respiratory tract and middle earinfections. Little is known about the pathogenesis of M. catarrhalis infection. A pathogenicbacterium requires adherence, invasion, establishment and dissemination with the host for asuccessful infection. The interaction between the host and the pathogen also includes uptakeand secretion of substances that are facilitated by a family of proteins termed transporters.ATP-binding cassette (ABC) transporter is one of the active transport system common inprokaryotic and eukaryotic cells. In this study, we identified a gene encoding ATPasecomponent of the ABC type transporter in M. catarrhalis and performed a nucleotide anddeduced amino acid sequence analysis of this gene. We used Genomic Survey Sequenceapproach (GSS) to identify the gene from M. catarrhalis genomic library. The insert size of thepositive clone is 934 bp and was sequenced by DNA walking. The ATPase domain of an ABCtype transporter has conserved motifs: Walker A, Q loop, C loop (signature sequence), WalkerB, D loop and Switch II. The ATPase domain is considered as the most conserved domain ofthe ABC type transporter due to presence of these motifs. Based on the homology and motifsearch, and amino acid sequence alignment, performed by using BLAST programs available onthe internet, it was found that the deduced amino acid sequence encoded by this gene exhibited50-80% identity with the sequences of the ATPase component of the ABC transporters ofseveral pathogens.Keywords: Moraxella catarrhalis, ABC type transporter, ATP-binding cassette transporter,ATPase domain, Walker A, Walker B.2