Immobilization of Mucor miehei and Pseudomonas sp. lipases onto florisil via glutaraldehyde and polysuccinimide and their uses in ester synthesis

In this study, the optimum immobilization conditions and the conditions in which free and 4 types of immobilized lipases showed their maximum activities and kinetic parameters were determined for immobilization of Pseudomonas sp. and Mucor meihei lipases onto activated florisil via glutaraldehyde and polysuccinimide spacer arms. The synthetic activities of immobilized lipases were also determined for the synthetic reactions such as L-ascorbyl palmitate synthesis and enantiopure amino alcohol synthesis and asymmetric amidation of racemic mandelic acid.It was found that the highest catalytic efficiency (kcat/Km) in terms of hydrolytic activity was determined as 6.91x105 s-1M-1 for Pseudomonas sp. lipase immobilized onto florisil via polysuccinime spacer arm among free and immobilized lipases. It was obtained about 90% yield in the synthesis of L-ascorbyl palmitate for all immobilized lipases under predetermined optimum conditions. It was found that all immobilized lipases were achieved the kinetic resolution of amino alcohols with 45-50% yield and 99% enantiomeric excess.It was determined that Pseudomonas sp. lipases immobilized onto florisil via glutaraldehyde and polysuccinimide both were achieved the (R)-Mandelamide synthesis with 50% yield and 99% enantiomeric excess.