The surveillance of mycobacterium bovis isolates with MIRU - VNTR and spoligotyping

To determine the epidemiological characteristics of M. bovis strains, isolated from human and animal tuberculosis cases, will be minimized by controlling a group of tuberculosis cases which is the source of animals and animal products and with effective control measures. In this study, It was aimed the investigation of M. bovis strains isolated from sputum samples of patients with pulmonary tuberculosis in Çukurova region and lungs and lymph nodes of slaughtered animals for meat production in Adana slaughterhouse on clonal level and determination of separation forces of methods used for this purpose, either alone or together, to be evidence of a possible transmission.Thirty-two M. bovis isolates of animal origin and 10 of human origin, isolated and identified between March 2011-June 2012, were used. 12 locus MIRU-VNTR and spoligotyping method was used for determining the clonal relationship. Six different pattern by spoligotyping and 10 by MIRU-VNTR was determined. When both methods were used together, the number of pattern was found to be 28 and MIRU4, MIRU26, MIRU31, MIRU40 discrimination power was the highest loci. The isolates concantrated in SB0120 pattern in the rate of 42.85 % in spoligotyping. By the same method, it was seen of 7 isolates were M bovis ssp caprae pattern and of 2 human isolates wereM. bovis BCG pattern. Neverthless, spoligotyping and MIRU-VNTR patterns were competible with isolates originated from cattle. The use of spoligotyping and MIRU-VNTR methods together was found more sensitive in epidemiological analysis of M. bovis isolates when compared to the use of either method alone. It is concluded that strains, sourced from same source in our region, infect both humans and animals. Therefore, continuation of studies on the molecular epidemiology of M. bovis and would be appropriate to share with the Ministry of Food Agriculture and Livestock.