Applications, characterization and integration of biological molecules with nanostructured materials

Producing biomaterials in nanoscale architecture is one of the most popular topic as biological and nanotechnologic applications particularly relies on biocompatibility and biodegradability. Among other biomaterials, carbohydrate-based polymers satisfy the demand in terms of availability, cost-efficiency and physicochemical properties. Electrospinning is a promising and simple technique to produce carbohydrate-based nanofibers. In this study, one of the cellulose derivative cellulose acetate (CA) was blended with chitosan (CS) and CA-CS nanofibers were synthesized via electrospinning technique. Different solvent systems and CA-CS polymer ratio were examined and process parameters were optimized to obtain the ultrafine and bead-free CA-CS nanofibers. Scanning electron microscopy (SEM) was performed to characterize the surface morphology of CA-CS nanofibers. Then, to test the CA-CS nanofibers as an immobilization material; glucose oxidase (GOx) was immobilized on CA-CS coated GCE by covalent modification to design biosensor platform. CV, DPV, EIS measurements were carried out to prove the surface modification and CA-CS/GOx biosensor was tested for glucose detection by amperometric technique. Limit of detection (LOD) and linear response for glucose of CA-CS/GOx biosensor was found 14 μM and 5 µM-0,75 mM respectively. Finally, CA-CS/GOx platform was investigated to detect glucose in synthetic samples.