Nazal yol yoluyla in situ jel yüklenen metoklopramid nano yapılı lipid taşıyıcı: Hazırlama, karakterizasyon ve in vitro değerlendirme

This study is done to overcome the limitations of MTC occurred when taken by different routes of administration in order to improve its bioavailability and to increase patient compliance, also in an attempt to prolong their nasal cavity residence time when it is taken intranasally, and for the achievement of a prolong drug permanence into the site of administration. An innovator composite formulates, by associating the liposome (soybean and cholesterol) and in situ gel (gellan gum) delivery systems, has been created. Thin film hydration method, also called bangham method was used to prepare MTC loaded liposomes. The optimal liposomal formulation was determined using D-optimal mixture experimental design. All of the prepared formulations, there values of polydispersity index were lower than 0.6, which indicates monomodal size distributions. Also, the particle size of the MTC loaded liposomes were small, the formulation's mean particle was smaller than 150 nm. The encapsulation efficiency of MTC in liposomal formulation was between 71% and 94%. Furthermore, in this work, zeta potential values were negative for all our API-Lip formulations. In the present study, MTC loaded liposome were prepared and incorporated with gellan gum solution. Furthermore, MTC loaded in situ gel preparation and characterization studies were performed, in addition to liposomal morphology, determination of mechanical properties, rheological behavior, ex vivo and in vitro release study. Based on observed data the MTC loaded liposome formulation incorporated with gellan gum was showing significant results. Cumulative drug release from the MTC loaded liposome incorporated with gellan gum formulations after 5 h was found to be more than 80%. This study might point to the potential of an intranasal innovative composite formulation taken together, liposomes included the nasal in situ gel in terms ease of administration, drug dosing accuracy, good capability for reduction of the nasal mucociliary transport time with prolongation of residence time at the site of absorption, the avoidance of antiemetic drug's bitter taste and improved drug bioavailability. Therefore, it could be taken into consideration in pharmaceutical industries in the future. Keywords: In-situ gel, Liposomes, MTC loded liposomes, Mucoadhesion, Residence time.