An investigation of the effects on MDA, GSH, SOD and NOx levels of the rats exposed to nonylphenol toxication

SUMMARYAN INVESTIGATION OF THE EFFECTS OF TAURINE ON MDA, GSH, SOD andNOx LEVELS OF THE RATS EXPOSED TO NONYLPHENOL TOXICATIONYasemin SUNUCU KARAFAKIOĞLUToday?s life is under threat of various toxic-influential environmental and chemical substances.Nonylphenol (NP), surface tension reductive chemical, is considered to be one of those substances andis widely used in the industry. As a consequence of understanding antioxidants? role in preventingagainst toxic factors, it has become quite significant to determine which antioxidant is effectual andsafely at what dose on which events. Investigating the role of taurine, a powerful antioxidant, inpreventing nonylphenol bound oxidative stress in the blood samples was aimed in this study. 40?Wistar-albino? male rats weighed between 175 and 250 g and aged between 3 and 4 months wereused in the study. Experimental rats were equally distrubuted amongst five groups provided that eachhas 8 of them: Control Group, Taurine Group, Nonylphenol Group, Nonylphenol + Taurine Group,and Alcohol Group since nonylphenol was treated and dissolved in the alcohol. As for piloting,nonylphenol stock solution including 1 μg nonylphenol in 1μl was prepared in the alcohol and thesolution which has 50 μg/kg nonylphenol was added through spraying into the diets of the rats placedin the Nonylphenol and Nonylphenol+Taurine Groups. Only 50 μl alcohol was added throughspraying into the diets of the Alcohol Group. Besides, taurin at the rate of 3 % (v/w) was added to thedrinking water of the Taurine and Nonylphenol+Taurine Group. MDA as a marker of lipitperoxidation appeared to increase (P<0.05) in all the experiment groups in proportion to the controls.However, the MDA levels in the rats treated only with taurine and treated with taurine in addition tononylphenol appeared to be lower statistically than the MDA levels in the rats treated only withnonylphenol and treated merely with alcohol (P<0.05). GSH results indicated that GSH levels in theNonylphenol, Nonylphenol+Taurine and Alcohol Groups decreased meaningfully (P<0.001) comparedwith that of the Control Group. Whereas, GSH levels in the Taurine Group appeared to be higher thanthat of the controls (P<0.001). When the results for the SOD levels are taken into consideration, theSOD level for the Nonylphenol, Nonylphenol+Taurine and Alcohol Groups appeared to decreasemeaningfully in comparison to that of the Control Group (P<0.01). Nevertheless, the SOD levelseemed to rise meaningfully in the group treated merely with taurine (P<0.01). The NOx levels werereduced amongst the rats treated with nonylphenol (P<0.01). The NOx levels within the groups treatedonly with alcohol and taurine and in the Nonylphenol Group along with taurine givenNonylphenol+Taurine Group appeared to be higher than that of the Control Group (P<0.01).As for conclusion, it has become apparent to apply antioxidants such as taurine in order to strengthenthe antioxidant defense wall while preventing oxidative stress induced by industrial chemicals such asnonylphenol. Moreover, it became clear that further studies to be conducted in this area in order todetermine the most influential antioxidant substance and its safely doses.Keywords : Oxidative Stress, Taurine, Nonylphenol, Toxication, Environment, Free Radicals,Antioxidants.