The determination of personal at the Nuclear Medicine area applications of radiation affect by biological dosimetry

ABSTRACTIn this thesis, the total effect of ionic radiation and chemical agent which staff who study inthe area nuclear medicine was exposed to has been observed through micronucleus (MN)analytical technique which was a biological dosimetry method.Micronucleus analysis method is one of the most widely used cytogenetic techniques fordetermining the chromosome defects in lymphocytes. Because MNi are expressed in cells thathave completed nuclear division, they are ideally scored in the binucleated stage of the cellcycle. Micronuclei (MNi) are small, spherical structure in cytoplasm, outside the mainnucleus but demonstrating the same shape and structure, but smaller than the main nuclei inthe cell, and painting properties with the nucleus.With this purpose, the blood samples taken from the employees working in the nuclearmedical centers were cultured according to the routine technique. After this, their MNcounting was performed and the dosage value gained in return of the activity transferred in tothe body was determined.In the direction of the results obtained in this study, the result that MN technique, which isused for human blood lymphocytes in literature, is an easy and sensitive method for studyingcytogenetic effects has been reinforced.Keywords: Biological dosimetry, micronucleus (MN)