Vascular effects of cape on isolated thoracic aorta of overiectomized rats

Aim: The aim of the study investigate the effect of CAPE on isolated thoracic aorta of menopausal model.Methods : Fifty ovariectomized Wistar- Albino rats randomly divided into eight groups (n=6-7) ; CAPE 10 ?M endothelium + group (I), CAPE 10 ?M endothelium - group (II), CAPE 100 ?M endothelium + group (III), CAPE 100 ?M endothelium - group (IV), CAPE 300 ?M endothelium + group (V), CAPE 300 ?M endothelium - group (VI), ethanol endothelium + group (VII), ethanol endothelium - group (VIII ) [n.b., negative ? indicates groups with damaged endothelium]. Rats were anaesthetized using ketamin (50 mg /kg ) + ksilasin (5mg/kg ). Chest cavity was surgically exposed from the bottom left side of the sternum. The heart and the lungs were moved to left side to expose the thoracic aorta. The thoracic aorta and aortic arch were carefully extirpated and placed in dish that had Krebs-Henseleit solution. After the dissection of fatty and other tissues around the aorta, it was sliced into 3-5 mm rings. The rings were quickly hung into a heated organ bath that contained carbonated Krebs- Henseleit solution. Aorta rings were first stretched by 1 gram and then stretched to 4 gm in 1 gm increments in 10 minutes episodes. When the tension records on a computer monitor were stable, contraction response using NE 10-4 M and relaxation response using 0.1 ml Ach (10-4) were obtained. Finally the preparation was washed twice using Krebs-Henseleit solution and the experiment was initiated 45 minutes later. During the experiment 0.1 ml NE 10-4 M was delivered into each of the baths and contraction responses of the aorta were observed. Once stable plateau contraction level was reached, 0.1 ml of CAPE of in 10 ?M, 100 ?M, 300 ?M doses were delivered into each of the three groups of the baths (3x4). Thirty percent of ethanol that is used to dissolve CAPE was prepared as a vehicle group and delivered in 0.1 ml volumes into the baths.Results: There was a significant dose dependant increase in the relaxation response of the aorta rings with healthy and damaged endothelium. The response of the group that had damaged endothelium and received 10 ?M CAPE was significantly different from the response of the group that had healthy endothelium and received 300 ?M CAPE (p<0.001). Other groups did not however display any significance.Conclusions: In aorta rings with healthy endothelium that was taken from menopause model rats the best relaxation response was obtained using 300 ?M CAPE. We believe that this novel study will shed light into the next generation of studies in this field of study.Key words: CAPE, isolated organ baths, aorta, relaxation responses, menopauseAddress for correspondence: drserce@gmail.com