Biochemical insecticide resistance in Culex pipiens complex species that distributed Middle and Eastern Black Sea region

In this study, it was aimed to determine the mechanisms causing resistance and resistance against organochlorine, organophosphorus, carbamate and pyrethroid insecticides in Culex pipiens complex species distributed in Central and Eastern Black Sea Region. For this purpose, sampling was carried out of the complex species from 8 different provinces in the area. The gathered specimens were brought to laboratory and differentiated according to the project aims and colonization procedures were carried out. Resistance status were identified according to the World Health Organisation descriptive doses for colonized populations. Species identification of the field collected samples was carried out morphologically and complex members were distinguished. The activity changes of 3 different enzyme systems were determined annually to investigate of the biochemical basis of developing resistance. For this purpose, the activity values of the esterase, oxidase, glutathion-S-transferase enzymes of the samples were measured. Comparisons of the obtained values were made annually and the changes were statistically analyzed. As a result of insecticide resistance tests, it was determined that low level pyrethroid resistance showed variation and higher rates resistance of organophosphate, carbamate and DDT. On the biochemical basis of the resistance, it has been determined that the enzyme systems in the populations have a multifactorial effect at different rates. The structures that supports the resistance in the field; the incidence of insecticide usage in the area and the insecticide pressure in agricultural areas and concluded that the uniform insecticide applications would increase resistance and adversely affect the control.