Synthesis and characterization of ozonated oil nanoemulsions

Ozone is a reactive molecule that breaks down in aqueous media into oxygen. It is used for disinfection in high concentrations in clinical applications and in accelerating wound healing and epithelization in low concentrations. The use of ozonated oil (ozone-enriched fat form) has been preferred in medical practice in recent years. The most important reasons for this preference are the high reactivity of the ozone and its effect on the intracellular signal system due to the compounds they form with unsaturated fatty acids. It is known that the ozone layer facilitates topical application of ozonetherapy, which can treat cutaneous and mucosal chronic infected areas. This M.Sc. thesis was financially supported by Bülent Ecevit University, Scientific Research Project number: 2015-39971044-02. The main purpose of this thesis is to synthesize the ozonated olive oil nanoemulsion system and examine the nanoemulsion production parameters using the emulsion inversion point (EIP) method. The oil, surfactant and water ratios used in the synthesis of nanomemulsion are optimized. However, the effects of surfactant-to-oil ratio, surfactant concentration and type, and mixing ratio on the mechanism of nanoemulsion formation have been examined. It has been found that high stability in the obtained nanoemulsions (~ 212.7 nm) can be produced at 750 rpm using tween 40 as surfactant. Antibacterial effects of the obtained nanoemulsions against Staphylococcus aureus and Escherichia coli were evaluated.