Pankreas adacıklarının ve nanoparçacıkların biyolojik işlev gösterebilen poli (etilen glikol) (PEG) hidrojeli ile kaplanması

Poly (ethylene glycol) (PEG) hydrogels have been extensively used in biomedical engineering, pharmaceutical applications, and biomaterial science due to their tunable structure, desirable mechanical features, high water content, and biocompatibility. In this study, the process of cell or nanoparticle coating through surface initiated photopolymerization is described. First, PEG hydrogel was synthesized on photoinitiator immobilized silicon surfaces, and physical properties such as swelling and thickness have been characterized. Next, surface initiated photopolymerization technique has been applied for the coating of pancreatic islets and magnetic iron oxide nanoparticles (MIONPs) within biomolecule functionalized PEG hydrogel, respectively. Viability and functionality assays demonstrated that islets coated within insulinotropic ligand functionalized PEG hydrogel have enhanced insulin secreting capability compared to control islets. Cell adhesion ligand functionalized PEG hydrogel coated MIONPs have also been investigated to assess their effect on HeLa cell viability and cellular uptake. These results suggest that the presence of cell adhesion domains within PEG hydrogel structure shows no toxicity to HeLa cells as well as improves cellular uptake of MIONPs by HeLa cells. In conclusion, coating of islets within functional PEG hydrogel before implantation may enhance functionality of transplanted islets and reduce the clinical transplantation volume, and coating of nanoparticles within functional PEG hydrogel may improve tumor detection as a result of enhanced MIONPs uptake by tumor cells.