CDC25A gene polymorphisim and expression in pankreatic cancer

The aim of this study, is to determine the relationship between CDC25A gene polymorphism and pancreatic cancer via determining CDC25A gene expression and polymorphisms of tumor tissues of pancreatic cancer cases. Additionally, it is aimed to prove the possible relationship between cancer development and the gene expression level with determining CDC25A gene expression levels in tumor tissue and normal tissue. For this purpose, we tried to make the literature data more comprehensive by determining CDC25A tissue expressions of 28 patients and blood polimorphisms of 118 patients.One hundred and eighty one patients (patients with pancreatic cancer who received surgery (n=28) and patients with pancreatic surgery who are in follow-up (n=90)) and as a control group, not having any known chronic disease, first degree relatives of not having cancer and healthy volunteers (n=83) were included in this study. Tissue and blood samples obtained from patients and blood samples from control group were applied for DNA and RNA isolation.There was no significant difference between the two groups; pancreatic cancer group and the control group CDC25A gene SER88PHE polymorphisms were compared according to C/C, C/T, T/T genotype frequencies and allele frequencies of G and A (p>0.05). There was no significant difference between the two groups; pancreatic cancer group and the control group CDC25A gene RS3731485 polymorphisms were compared according to C/C, C/G, G/G genotype frequencies and allele frequencies of C and G (p>0.05). CDC25A gene expression levels were studied on normal and tumor tissue specimens taken from patients during surgery. Reference Range was taken as high <1.5, low >0.5, 0.5Keyword: Ekspresyon = Expression ; Neoplazmlar = Neoplasms ; Pankreas = Pancreas ; Pankreas neoplazmları = Pancreatic neoplasms ; Polimorfizm-genetik = Polymorphism-genetic