Investigation of dose dependent biochemical effect of caffeic acid phenethyl ester on experimental oxidative stress generated by using paraquat i̇n a549 lung epithelial cell line

Paraquat (PQ) is a high toxic, quarternary nitrogen herbicide. It is widely used in the world due to its low price, rapid effect and safe. The main target organs affected by accidental or intentional PQ exposure are the lungs. In PQ toxicity, induction of reactive oxygen species takes an important place. Therefore, the use of antioxidants against the formation of induced reactive oxygen species is seen as an alternative treatment method.Caffeicacidphenethyl ester (CAPE) is a polyphenol found in some plants, which is also one of the active components of propolis produced by honeybees. CAPE is an effective molecule against infection, oxidative stress, inflammation, cancer, diabetes, neurodegeneration, and anxiety. In this thesis study, cell viability test (MTT), total oxidant capacity (TOC) analysis, total antioxidant capacity (TAC) determination and oxidative stress index (OSI) were calculated in order to determine the effect of caffeic acid phenethyl ester on oxidative stress induced by PQ in A549 lung epithelial cells. For this purpose, 400 M paraquat and 1; 2, 5 and 5 g/ml CAPE were applied to A549 cell lines for 24 hours. At the end of the application, it was determined that PQ significantly decreased cell viability, increased TOC values and decreased TAC values. It was found that cell viability was increased in the groups of 1 g and 2, 5g/ml of CAPE together with paraquat compared to the groups treated with paraquat alone, whereas 5 g/ml CAPE had no curative effect on cell viability. TOC values were found to be lower in CAPE group compared to PQ group, but remained a slighty statistical difference. In the PQ group, decreasing TAC values increased significantly with 2, 5g/ml CAPE application, while 5 g/ml CAPE application reduced TAC values compared to the control group. In the 1 g/ml CAPE group, the TAC values were higher than the PQ group but showed a slighty statistical difference. OSI values were higher in the PQ and PQ + 5 g/ml CAPE groups than in the control group and lower in the PQ + 2.5 g/ml CAPE group than the PQ group. Based on these findings, it can be said that caffeic acid phenethyl ester is dose-dependent and has protective or therapeutic properties against paraquat induced oxidative stress damage.