Peptit biyobelirteç tespiti için moleküler baskili polimer bazli biyosensör sistemi

Biosensors for a range of targets would be more crucial as indicators of disease, health status, environmental monitoring, food quality, fermentation control, and civil defense applications grow more widespread. One of the approaches being researched to enhance their unique recognition properties is molecularly imprinted polymers (MIPs). With exceptional sensitivity and selectivity, MIPs mimics the structure and binding principle of antibodies and biological receptors. MIP is a key component in constructing polymeric sensors and can be employed in various signal amplification or transmission systems. Molecularly imprinted polymers have generally been used for small molecules, but have limited uses for large molecules such as peptides. Detection and early diagnosis of peptide biomarkers is important in medicine and many other fields in order to prevent peptide-related diseases such as thrombin deficiency, which is an extremely rare bleeding disorder. In this study, the detection of various peptide molecules was made using MIP, and it was proven once again that molecular imprinted polymers are useful for large molecules such as peptide molecules. In this study, MIPs were created and studied using different polymers for a peptide biomarker. Based on the results of the experiments without aptamer, it was concluded that the studies using polydopamine were more stable and more selective, and the system was developed as a dual hybrid based on MWCNTs/Aptamer/MIP. During the MIP preparation steps, several monomers were used to test the detection of thrombin, an exemplary peptide biomarker. Electrochemical methods such as CV, DPV, EIS were used to evaluate the effectiveness of different polymers and the results were evaluated. And again, cyclic voltammetry, electrochemical impedance spectroscopy and differential pulse voltammetry were used to characterize the MIP-based sensor system. The MIP-based biosensor device for thrombin has a detection limit of 2.5 pg/mL and a robust linear response from 10 pg/mL to 0.5 ng/mL.