Peri i̇mplant defektlerde D vitamininin osseointegrasyona etkisinin histolojik ve histomorfometrik olarak incelenmesi

The aim of this study was to investigate the effect of sustained-release vitamin D loaded in the poly lactic acid (PLA) nanoparticles and applied with grafting materials around titanium (ti) dental implants. A total of 48 implants were inserted into the standardised bone defects created in the ilium 6 sheep and seperated to four different experimetal sites (empty control, autografted, xenografted and autograft+ xenografted). Vitamin D3 which is encapsulated by poly-lactic acid (PLA) nanoparticles were prepared and applied in half of the defects which left to heal for 3 and 6 weeks. New bone formation (NBF%) and bone-implant contact (BIC%) values were evaluated by histologic and histomorphometric analyses (p<0.05). Nanoparticles with a yield of 80,30% ± 2,14 and a high encapsulation efficiency of 72,99 % ± 2,20 were achieved. In all sections, osseointegration was observed and there were no signs of inflammation, necrosis or foreign body reaction. NBF and BIC% were significantly higher in vitamin D3 applied groups (p<0.001). In both healing periods, highest NBF nad BIC% was recorded in the autograft+xenografted groups (For the respective 3rd and 6th weeks; 49.63±1.53 and 74.25±0.96 NBF% and 54.26±0.66 and 82.59±2.09 BIC%; p<0.001). Vitamin D3 loaded sustained release PLA nanoparticles improves NBF and BIC% around ti implants and warrants further investigation for its clinical applicability.