Prosopis farcta (çeti) bitkisinin meyve kısmının antioksidan kapasitesi ve flavonoid içeriği

Free radical is an atom, molecule or ion with an unpaired electron. Although having important role in energy circumtances for cells, oxygen association with free radicals cause oxidative stress.Oxidative stress can be defined as unbalancaed reduction and oxidation situation which damages cell structure and cause many diseases. For instance; heart and cardiovascular diseases, cancer, immune system related diseases and aging are occured by oxidative stress. Not only effective in human cells but also oxidative stress cause degradation of vegetables and fruits. Antioxidants are compounds, which react with reactive oxygen and nitrogen species and free radicals. Free radicals reaction cause many disease in human body, likewise all type cancer or heart disease. On the other hand, free radical reaction cause deterariton on foods, cosmetic products etc. Considering harmfull effect of free radicals reactions, antioxidant compounds are important for healthy life, cosmetic industry and food industry. In wiev of the fact that with globalization and increasing interest in green chemistry, natural antioxidants source became more important. Considering synthetic antioxidant usage restricted for their toxic effects in many countries, natural antioxidants source investigation have been increased. Natural antioxidants found in plants, fruits and vegetables which are rich in antioxidant compounds. Flavonoids, caretenoids and phenolics are plant source antioxidants. Flavonoids are secondary metabolites of plants which can be classified as natural compounds. Flavonoids groups have common naturş antioxidant compound that commonly used like apigenin, myricetin etc. Prosopis farcta is a small, prickly shrub, 30-80 cm tall species of prosopis and widespread in Northern Africa Asia and west to the Middle East. In Turkey, Prosopis Farcta only found in Şanlıurfa area. Both in Turkey and others populated Prosopis species have long traditional usage by local people. Prosopis Farcta, which collected in Şanlıurfa used for diabetic diseases as making tea. Besides using as tea, Prosopis facta mostly seen as problem by farmers because it has strong seeds. Due to this reason, P.farcta has forgotten usage and less assays in Turkey. On the other hand, global assays show that high degree of salt toleration, having effect on cholesterol and diabetes hurts are P.farcta's important properties. In vıvo works show that P. farcta accelerates healing cutaneous wounds in diabetic rats. On the other hand, there are some acedemic works for P.farcta's effect on relaxition of rat's aorta. Besides these effects, it's antioxidant activity, antiparasitic and antimicrobial have been noted 21 recently. There is no report to show an academic research to antioxidant effect of this plant so far. Flavonoid diversification of Prosopis farcta occurring two different places shows different percentage and different diversification. Having considering traditional usage, flavonoid diversity of Turkey's Prosopis farcta is important research issue. In this study, antioxidant capacity of Prosopis farcta beans examine by MODIFIED CERAC, CUPRAC, MODIFIED FOLIN methods. Prosopis farcta that have been collected Şanlıurfa, storaged proper conditions in laboratory. Both infusion extraction and methanol extraction solutions are prepared by pericarps of storaged Prosopis Farcta. For antioxidant capacity analysis three different methods applied to both methanol solutions and infusion solutions. Different methods which are MODIFIED CERAC, CUPRAC and MODIFIED FOLIN applied due to having different advantages and disadvanteges against eachother. Therefore correlation in results can obtained in this study. HPLC system used for identification of Prosopis farcta bean's flavonoid diversity. According to flavonoid diversity of Prosopis farcta have not been reported, proper parametres of HPLC system decided by several experiments. Chromatographic analysis applied to methanol solutions of prosopis farcta. First, main peaks of Prosopis Farcta solution chromatograms decided. Then,chromatograms of standards solution database formed. Flavonoid diversity of prosopis farcta are identified with standard addition methods by comparing chromatograms. According to results of this study, differences between infusion and methanol/water extraction solution observed in antioxidant capacity assays.Samples of infusion solution has higher antioxidant capacity than methanol/water extraction samples. Due to qualitative and quantitative results, based on HPLC, flavonoids diversity show differance from Tunusia populated Prosopis farcta beans. Quercetin, cafeic acid and gallic acid are found in beans of P.Farcta populated in TURKEY. On the other hand, base peaks of Prosopis farcta beans couldn't identified in this study.