Karyotype properties of Pseudophoxinus firati (Pisces: Cyprinidae)

This study was carried out on the chromosomal features of samples belonging to the Pseudophoxinus firati Bogutskaya, Küçük & Atalay, 2006 (Pisces: Cyprinidae) collected from Tohma Çayı (Sivas Province, Gürün district, Yazyurdu village) by cytogenetic studies (karyotype, C-banding and nucleolus organizer region-NOR). Air drying technique described by Collares-Pereira (1992) was used for karyotype analysis. Technique of Sumner (1972) was used for C-banding of sample preparations, whereas the a 1-step technique of Howell and Black (1980) was used for silver staining. The diploid chromosome complement was 2n= 50, consisting of 19 pairs of meta-submetacentric, 6 pairs of subtelocentric and NF=88. Sex chromosomes were morphologically undifferentiated. Constitutive heterochromatin region with C-banding was determined on the 6 pairs of chromosomes. NOR was observed on the short arms of 2 pairs of medium sized submeta-subtelocentric chromosomes. There was no chromosomal study on P. firati, thus this study may contribute to cytogenetic and taxonomy of this and related species.