Effect of benzo(A)pyrene on the homocysteine level and lipid parameters in rats

In this study, investigation of effects of administration of benzo(a)piren (BaP) to rats on homocysteine level, lipid parameters and coronary arteries was intended. For this purpose, approximately 200-250 gr. in Weight, 8-10 weeks age, 17 wistar albino female rats were divided into two groups. These groups; 1st Group (Control Group, tricapyrilin as placebo), and 2nd Group ( BaP Group: 3,4 benzo(a)piren dissolved inthe tricapyrilin) administrated BaP10 mg single dose with subcutaneously.10 weeks after application and after 12 hours hunger period, blood samples were taken from heart with punction under ether aneshesthesia. Taken blood samples were investigated for plasma homocysteine, total cholesterol, triglycerides, LDL, VLDL and HDL levels and coronary arteries were investigated for pathological changes. According to results; despite there was difference found on total cholesterol, triglycerides LDL, VLDL and HDL levels of BaP group statistically (p<0,05), there was no significant difference between homocysteine levels (p>0,05). There was no significant differences were detected on mean blood vessel diameter and blood vessel wall thickness value between groups (p>0,05). In experiment group, vacuolar degeneration and significant increase in peri-arteriolar connectivite tissue of muscle cells in the muscular layer of epicardial coronory arteries. As a result; we believe determined effects of BaP on lipid parameters and homocysteine level will make a significant contribution to understanding etiology of cardiovascular diseases. Key Words: Homocysteine, Benzo(a)pyrene, BaP, Atherosclerosis, Lipid Parameters.