Experimantal heart attack and injury model in rats and testing different treatment methods

One of the diseases with the highest mortality rate in our country and in the world is heart attack. In order to correct this situation where many risk factors are effective, to increase the quality of life of the society, to reduce the morbidity and mortality rates, and most importantly, studies are continuing intensively in different fields for the treatment of heart attack, also known as myocardial infarction, so that people can access treatment in the most effective, fastest, most economical way and in the most accessible way. In this study, it is aimed to induce a heart attack by experimentally giving the active substance (isopretrenol) to rats and to cause myocardial damage by adding homocysteine to the drinking water of the rats. It has been targeted to investigate the effects of these substances against heart attack and myocardial damage by using therapeutic active substances (euganol, resveratrol, garlik extract) following the applications. According to the positive results by making genetic examinations, it has also been tested for use on humans, so that in addition to known methods, the protection of resveratrol, euganol and garlic extract against heart attack and myocardial damage has also been tested, and it is aimed to demonstrate the effectiveness of these substances in alternative medicine in repairing DNA damage that may lead to disease. The effect levels of isopretrenol, which is used to cause heart attack, and homocysteine, which is used for myocardial damage, are taken into account as beta actin gene control gene; By applying PCR, RT-PCR, gene expression levels were examined, caspase-3 gene and cytochrome-c levels were examined, and apoptosis levels were investigated. Application of resveratrol, euganol, homocysteine used for therapeutic purposes; The applicability and level of effectiveness were also determined by this method, and biochemically, ck (creatinine kinase) ,ckmb (ck-2) , troponin-t, LDL, Lactate Dehydrogenase parameters were measured and the changes made by the applied methods were determined. Pathological changes were also evaluated using samples iv taken from myocardial tissue after a heart attack. When all the findings of the study are evaluated and the results are examinedthe following results were obtained; Regarding heart attack and damage, experimentally administered isopretrenol causes heart attack and homocysteine causes myocardial damage, the treatment of active substances (resveratrol, garlic extract and euganol) given for therapeutic purposes has a protective and therapeutic effect at different doses and levels, suppresses apoptosis and observing its effects on cytochrome c activity and cas- activity, as a complementary treatment method, all of these active substances are effective, albeit in different ways and on different markers. Keywords: Heart Attack, Isopretrenol, Euganol, Resveratrol, Homocystein, Garlic