Investigation of the effects of olive leaf extract on acute intestinal ischemia-reperfusion injury in rats

In this thesis on the investigation of the effects of olive leaf extract on acute intestinal ischemia reperfusion injury in rats, the effectiveness of olive leaf extract on acute intestinal ischemia reperfusion injury in rats is investigated, the effects of olive leaf extract on preventing mesenteric ischemia injury are investigated, and this study is used in the future to shed light on the effects of olive leaf extract on ischemia. Wistar male albino rats weighing between 250-300 g were used in this study. In the study, 32 rats were randomly selected, each experimental group consisting of 8 male rats, and the study was started after receiving ethics committee approval, 4 groups in total (n = 8). Rats were housed in a 12-hour light, 12-hour dark environment, in standard cages and in the same laboratory. After the rats started working, they were given ad libitum rat food and allowed to drink water freely for up to 12 hours before the operation. Groups; They were classified as control, SHAM, IIR-200 and IIR-400. In the control group, only healthy rats were fed. Only acute intestinal ischemia reperfusion injury occurred in the rats in the SHAM group. Acute intestinal reperfusion injury was created after the rats in the IIR-200 group were given olive leaf extract at a dose of 200 mg/kg by oral gavage once a day for a total of 7 days. Acute intestinal ischemia reperfusion injury was caused to the rats in the IIR-400 group after they were given olive leaf extract at a dose of 400 mg/kg via oral gavage once a day for a total of 7 days. In the SHAM, IIR-200 and IIR-400 groups, under deep anesthesia, the abdomen was prepared for laparotomy under sterile conditions, median laparotomy was performed, and the arteria mesenterica cranialis from the cranial arteria renalis was occluded with bulldog clamps for 30 minutes, and at the end of 30 minutes, the clamp was removed and reperfusion was performed for 2 hours. The cases were sacrificed at the 24th hour. Samples were collected from the ileum of sacrificed rats. The sacrification process was performed with high doses of anesthetic. Blood samples were taken by cardiac puncture. When the ileum samples taken were examined histopathologically, the results were recorded as 0.38±0.38, 2.75±0.16, 2.13±0.13, 0.50±0.19 in the groups, respectively. When the findings obtained in all groups were compared statistically, it was determined that there were statistical differences between the control and sham and IIR-200 and IIR-400 groups (P < 0.05). Hemogram values and serum TAS, TOS, IL-1, IL-6, TNF-α, HYP, NO were measured in the blood samples taken, when the IIR-200 and IIR-400 olive leaf extract groups were compared with the SHAM and control groups, TAS was biochemically determined to be higher in the IIR-400 group and lower in the IIR-200 group (P<0.05). However, proinflammatory cytokines were determined to be at lower levels (P<0.05). HYP, on the other hand, was determined to be arithmetically lower when comparing the control and SHAM groups with the IIR-400 group, although it did not have statistical significance (P>0.05). Again, NO was measured at lower levels in the SHAM group compared to the IIR-400 group (P<0.05). Based on the data in the study, it was seen that olive leaf extract had positive effects in preventing ischemia.